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Progesterone is an essential regulator of the reproductive events associated with the establishment and maintenance of pregnancy, including ovulation and uterine & mammary gland development. Progesterone exerts its effects via the intracellular progesterone receptor (PR). PR is composed of two isoforms, A and B. The B form of PR acts as a transcriptional activator in different cellular contexts, whereas the A form functions as a strong inhibitor of transcriptional activity. Quantitative detection of PR levels is very important for predicting prognosis and evaluating the outcome of endocrine therapy in patients with breast and/or uterine cancer.
Figure 1: Monitoring protein expression levels of PR using NR Sandwich.
Different amounts of nuclear extracts from two different breast cancer cell lines: T-47D and MDA-MB-231 were analyzed for levels of total PR protein using the NR Sandwich PR Kit. This data is provided for demonstration purposes only.
Applications
The NR Sandwich PR has many applications including:
- Study of PR transcriptional activity regulation
- Protein structure/function studies of PR and its mutated variants in areas such as osteoporosis, arteriosclerosis and breast cancer
