YOUR CART
Sequence-specific gripNA™* probes provide researchers with the very latest technology available for gene silencing. This powerful new tool utilizes the high-affinity binding and sequence specificity of engineered peptide nucleic acids (PNAs) to improve gene targeting and reduce non-specific interactions. Active Motif’s custom gripNA synthesis service will accelerate your research by providing you with a unique alternative to the more traditional techniques used for gene silencing. Note: because each gripNAs order is a custom synthesis of the sequence you choose, to order you must complete and fax a copy of the gripNA Fax Order Form (Documents tab).
gripNA advantages
- Unsurpassed sequence specificity
- Resistant to nuclease degradation
- Easy delivery with Chariot™ II
- Flexible synthesis modifications
- Highly soluble
* Covered under U.S. Patent No. 6,962,906. Purchase includes the right to use for basic research only. Other-use licenses available, please contact your local Technical Services dept.
Scale of Synthesis
gripNAs are synthesized at a 200-nmol scale. This is, on average, sufficient for over 600 cell culture experiments carried out in 12-well plates. The number of experiments will increase or decrease depending on the size of the culture plate used and the amount of gripNA used per experiment.
Purification
gripNA oligos are purified using cartridge purification; a reverse phase chromatography procedure ensures that synthesis impurities, ammonia and truncated sequences are eliminated from the final product. The gripNA is then desalted and lyophilized.
Quality Control
To ensure the quality of gripNA probes, every gripNA is analyzed by (MALDI-TOF) mass spectrophotometry. This technique provides a fast, accurate method of determining the mass of an oligonucleotide and can determine the presence of unwanted species, such as deletion sequences, depurination product and incompletely deprotected.
Chariot II for gripNA Delivery
When ordering your gripNA probe, you have the option of ordering Active Motif’s Chariot II. gripNA delivery into mammalian cells using Chariot II is simple and provides high transfection efficiencies with minimal cytotoxicity. Enough Chariot II is provided for ~96 gripNA deliveries in 12-well plates.
Other methods available for gripNA delivery into mammalian cells include lipofection and cell scraping methods, though these methods are more toxic and require substantially more gripNA per delivery, respectively. For delivery details, please download the gripNA manual.
Positive Control
One 5 nmol vial of 3´-Fluorescein-labeled human CREB gripNA is supplied free of charge with each 10 gripNA sequences ordered (i.e. 1 vial for 1-10 gripNAs, 2 vials for 11-20 gripNAs, etc.). Each vial is sufficient for ~16 cell culture experiments carried out in 12-well plates. If you would like to purchase additional vials of positive control, 25 nmol vials are available for purchase. Please fill in the number of additional vials you would like at the bottom of the order form.
gripNA Shipment
gripNAs are supplied lyophilized and can be shipped at room temperature. gripNA orders from within North America will be delivered within 21 days of Active Motif receiving the order. Orders originating outside North America may require additional time due to extra handling and customs.
Placing Your Order
In order to prevent errors, Active Motif will not accept phone orders for custom gripNAs. Customers must place their order by downloading the gripNA Fax Order Form, which is found under the Documents tab above. This form should be filled out and faxed to the appropriate Active Motif office or, if you are in a country served by a distributor, then faxed to your distributor.
