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p38 MAP kinase is a member of the Mitogen Activated Protein Kinase (MAPK) family of proteins. It was first identified in a screen for drugs inhibiting tumor necrosis factor-mediated inflammatory responses. p38 MAPK is activated in response to a variety of stimuli including growth hormones, ligands for G-protein coupled receptors, inflammatory cytokines and stresses such as osmotic shock and heat shock. Because of its critical role in inflammation regulation and stress response, p38 MAPK is of great interest in both basic and therapeutic research and high-throughput study methods are in demand.
Figure 1: Colorimetric measurement of phosphorylated p38.
Macrophage 4/4 cells were cultured in 96-well plates. Cells were induced with the indicated amount of lipopolysaccharide (LPS) for 20 minutes. Phosphorylation of p38 was assayed in triplicate using the FACE p38 Kit. Cell numbers in each well were normalized using Crystal Violet.
Antibody Specificities
The phospho-p38 antibody is specific for phosphorylated p38 and was raised against a peptide phosphorylated on residues that correspond to the sequence surrounding Thr180 and Tyr182 of human p38. It recognizes p38 alpha, beta, gamma and delta isoforms. The total-p38 antibody recognizes p38 MAPK regardless of its phosphorylation state.
