YOUR CART
p38 MAP kinase is a member of the Mitogen Activated Protein Kinase (MAPK) family of proteins. It was first identified in a screen for drugs inhibiting tumor necrosis factor-mediated inflammatory responses. p38 MAPK is activated in response to a variety of stimuli including growth hormones, ligands for G-protein coupled receptors, inflammatory cytokines and stresses such as osmotic shock and heat shock. Because of its critical role in inflammation regulation and stress response, p38 MAPK is of great interest in both basic and therapeutic research and high-throughput study methods are in demand.
The FACE™ Method
In FACE, cells are cultured in 96-well plates and stimulated to induce the pathway of interest. Following stimulation, the cells are fixed rapidly, which preserves activation-specific protein modifications. Each well is then incubated with a primary antibody specific for the activated protein of interest. Subsequent incubation with secondary HRP-conjugated antibody and developing solution provides a colorimetric or chemiluminescent readout that is quantitative and reproducible (Figure 1). The number of cells in each well can be normalized easily with the provided Crystal Violet solution. FACE Kits also contain primary antibody specific for the native inactive protein, so you can monitor both native and activated protein levels in the same experiment. FACE eliminates cellular extractions, radioactive kinase assays, time-consuming Westerns and inefficient epitope interactions that occur on membranes. FACE is a highly sensitive high-throughput assay designed for detecting activated proteins within mammalian cells.
