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Chariot™* is a revolutionary delivery reagent that quickly and efficiently transports biologically active proteins, peptides and antibodies directly into cells. The typical delivery efficiency is 60-95%. Less than two hours after delivery, live cells can be assayed to determine the effects of the introduced material, without the need for fixing. This makes Chariot an ideal tool for functional studies, including delivering inhibitory proteins, labeling organelles, screening peptide libraries and studying protein half-lives & transient complementation.
* Chariot is covered under U.S. Patent No. 6,841,535. Purchase includes the right to use for basic research purposes only. Other use licenses are available, please contact Active Motif Technical Service for additional information.
Contents & Storage
Chariot is offered in 25- and 100-reaction kits, where a reaction is defined as the amount of reagents required to deliver the β-galactosidase positive control protein to cells in one well of a 6-well plate or in a 35 mm plate. The number of reactions you can perform will vary depending on the size plates used and the type of macromolecule(s) delivered. Each Chariot Kit contains sufficient Chariot Transfection Reagent, PBS, β-galactosidase positive control protein and sterile water for either 25 or 100 reactions. Store all reagents at -20°C. All reagents are guaranteed stable for 6 months.
