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The β-Galactosidase Staining Kit is an easy-to-use and efficient method to determine the percentage of cells expressing lacZ following transient or stable transfection of plasmids that contain lacZ.
The gene product of lacZ, β-galactosidase, catalyzes the hydrolysis of X-gal, which produces a blue color that is easily visualized. The kit can also be used to determine the efficiency of direct delivery of β-galactosidase by the Chariot™ Protein Delivery Reagent (Figure 1). It is also suitable for assaying tissue sections after in vivo transfection.
Figure 1: Staining of HeLa cells.
1 µg of a 119 kDa subunit of β-galactosidase was complexed with Chariot™ Protein Delivery Reagent for 30 minutes and delivered into HeLa cells. Two hours post-delivery, cells were fixed and stained using the β-Galactosidase Staining Kit.
