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The Suspension Cell FACE module was designed to be used with any colorimetric or chemiluminescent FACE Kit; it provides you with 96-well filter plates that make it easier to perform washing & liquid handling steps through use of an appropriate vacuum manifold.
Simply substitute the filter tissue culture plates provided in Suspension Cell FACE for the standard 96-well plates supplied with FACE Kits, and use the Suspension Cell Blocking Buffer in place of the Antibody Blocking Buffer. In addition, fluorescent Hoechst stain is included for normalizing results, as the Crystal Violet stain included in FACE Kits stains the filters of the 96-well filter plates.
Figure 1: FACE ERK1/2 with the Suspension Cell FACE module.
The Suspension Cell FACE module was used with FACE ERK1/2 Chemi to assay 50,000 Jurkat cells per well. Jurkat cells were serum-starved and treated with 100 ng/ml PMA (Phorbol 12-myristate 13-acetate) for 15 minutes. A three-fold increase in phospho-ERK1/2 was detected in the assay.
