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HDAC Assay Kits

colorimetric or fluorescent assays for histone deacetylase activity

HDAC Assay Kits are 96-well plate-based assays to determine the HDAC (Histone Deacetylase) activity in your extracts or purified samples. HDAC Assay Kits are available in both colorimetric and fluorescent formats to suit your needs.

 
Name Format Cat No. Price  
HDAC Assay Kit (Fluorescent) 1 x 96 rxns 56200 $420 Buy Now
HDAC Assay Kit (Colorimetric) 1 x 96 rxns 56210 $395 Buy Now

Each HDAC Assay Kit contains positive control HeLa nuclear extract, deacetylated HDAC assay standard, HDAC peptide substrate, Trichostatin A as a model inhibitor and all the essential buffers for a full 96-well plate assay.

Assay advantages

  • Works with Class I, IIB and IV HDAC enzymes (does not work with HDAC Class IIA)
  • Easy to adapt to work with Class III HDAC enzymes
  • Use nuclear extracts, immunoprecipitates, column fractions or purified proteins
  • Calculate enzymatic activity and screen inhibitor compounds
 
Figure 1: Colorimetric HDAC Assay results.

HeLa nuclear extracts were assayed from 0 to 50 µg per well in duplicate. The purple line represents activity from untreated extracts, while the copper line represents extracts treated with 1 µM Trichostatin A inhibitor.

 
Figure 2: Fluorescent HDAC Assay results.

HeLa nuclear extracts were assayed from 0 to 10 µg per well in duplicate. The purple line represents activity from untreated extracts, while the copper line represents extracts treated with 1 µM Trichostatin A inhibitor.

Contents & Storage

HDAC Assay Buffer, HDAC Substrate, HDAC Assay Standard, Trichostatin A, HDAC Developer Solution, HeLa Nuclear Extract, 96-well half-volume plate. HeLa Nuclear Extract is to be stored at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.