HDAC Assay Kits
colorimetric or fluorescent assays for histone deacetylase activity
HDAC Assay Kits are 96-well plate-based assays to determine the HDAC (Histone Deacetylase) activity in your extracts or purified samples. HDAC Assay Kits are available in both colorimetric and fluorescent formats to suit your needs.
|HDAC Assay Kit (Fluorescent)||1 x 96 rxns||56200||$375||Buy Now|
|HDAC Assay Kit (Colorimetric)||1 x 96 rxns||56210||$375||Buy Now|
|HDAC Assay Kit (Colorimetric) Manual|
|HDAC Assay Kit (Fluorescent) Manual|
|HDAC Assay Kits Profile|
|Histone Analysis Products Profile|
|Histone Modifications Guide|
|Chromatin & Epigenetics Products Brochure|
Each HDAC Assay Kit contains positive control HeLa nuclear extract, deacetylated HDAC assay standard, HDAC peptide substrate, Trichostatin A as a model inhibitor and all the essential buffers for a full 96-well plate assay.
- Works with Class I, IIB and IV HDAC enzymes (does not work with HDAC Class IIA)
- Easy to adapt to work with Class III HDAC enzymes
- Use nuclear extracts, immunoprecipitates, column fractions or purified proteins
- Calculate enzymatic activity and screen inhibitor compounds
Figure 1: Colorimetric HDAC Assay results.
Figure 2: Fluorescent HDAC Assay results.
Contents & Storage
HDAC Assay Buffer, HDAC Substrate, HDAC Assay Standard, Trichostatin A, HDAC Developer Solution, HeLa Nuclear Extract, 96-well half-volume plate. HeLa Nuclear Extract is to be stored at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.