		Transcriptional Regulation Co-Immunoprecipitation DNA Repair Luciferase Reporter Assay Nuclear Receptor ELISAs Supershift & Gelshift Assays Transcription Factor ELISA Epigenetics & Chromatin Chromatin Assembly Chromatin IP DNA Damage – Phospho-H2AX DNA Methylation Histone Acetyltransferase Activity (HAT) Histone Deacetylase Activity (HDAC) Histone Purification Methylated Histones Protein Modification GTPase Activation Assay Kinase Assay Substrate Mitosis Assays Nitric Oxide Quantitation Phospho-Protein Detection Sandwich ELISAs SUMOylation Antibodies, Extracts & Proteins Antibodies Cell Extracts Recombinant Proteins Fluorescence & Labeling Albumin Quantification Cell Viability Assay Fluorescent Dyes & Labeling Immunofluorescence Live Cell Membrane Staining Mitosis Determination Protein and Extract Quantification Protein Labeling & Translocation Sample Preparation Fluorescent Dye Labeling Mitochondrial Separation mRNA Isolation Normalization Nuclear & Cytoplasmic Extraction Protein & Extract Quantification Transfection & Expression Competent E. coli Gene Silencing Protein Transfection Staining Reagents Bioinformatics & Text Mining TimeLogic® Biocomputing Solutions AKS 2

TransAM™ IRF-3 and IRF-7

Interferon Regulatory Factors (IRFs) are a family of transcription factors involved in host defense via regulation of anti-viral immune responses, cell growth and hematopoietic development. TransAM IRF-3 Kits are available for human and mouse or rat IRF-3, and the TransAM IRF-7 Kit is available for human IRF-7. The TransAM IRF Kits utilize antibodies specific for the active form of IRF-3 or IRF-7 when it is bound to the target DNA.

Figure 1: Monitoring IRF activation with the TransAM IRF-3 (Human) Kit.

0.6 to 10 µg of poly (I/C) treated COS-7 cells were tested for IRF-3 activity using the TransAM IRF-3 (Human) Kit.

Figure 2: Monitoring IRF activation with the TransAM IRF-7 Kit.

40 ng to 0.6 µg of IRF-7 transfected COS-7 cells were tested for IRF-7 activity using the TransAM IRF-7 Kit.

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TransAM™ IRF-3 and IRF-7

Figure 1: Monitoring IRF activation with the TransAM IRF-3 (Human) Kit.

Figure 2: Monitoring IRF activation with the TransAM IRF-7 Kit.

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