		Transcriptional Regulation Co-Immunoprecipitation DNA Repair Luciferase Reporter Assay Nuclear Receptor ELISAs Supershift & Gelshift Assays Transcription Factor ELISA Epigenetics & Chromatin Chromatin Assembly Chromatin IP DNA Damage – Phospho-H2AX DNA Methylation Histone Acetyltransferase Activity (HAT) Histone Deacetylase Activity (HDAC) Histone Modification ELISAs Histone Purification Methylated Histones Protein Modification GTPase Activation Assay Kinase Assay Substrate Mitosis Assays Nitric Oxide Quantitation Phospho-Protein Detection Sandwich ELISAs SUMOylation Antibodies, Extracts & Proteins Antibodies Cell Extracts Recombinant Proteins Fluorescence & Labeling Albumin Quantification Cell Viability Assay Fluorescent Dyes & Labeling Immunofluorescence Live Cell Membrane Staining Mitosis Determination Protein and Extract Quantification Protein Labeling & Translocation Sample Preparation Fluorescent Dye Labeling Mitochondrial Separation mRNA Isolation Normalization Nuclear & Cytoplasmic Extraction Protein & Extract Quantification Transfection & Expression Competent E. coli Gene Silencing Protein Transfection Staining Reagents Bioinformatics & Text Mining TimeLogic® Biocomputing Solutions AKS 2

TransAM™ SREBP1

Sterol-regulatory element (SRE)-binding proteins (SREBPs) belong to the family of basic helix-loop-helix leucine zipper transcription factors. SREBPs can be difficult to study since they are synthesized as inactive precursor proteins bound to the endoplasmic reticulum and nuclear envelope, yet once they are activated by sterol deprivation the precursor protein is cleaved to release the activated N-terminal portion. The cleaved SREBP1 then enters the nucleus and activates transcription of genes involved in cholesterol and fatty acid synthesis. TransAM SREBP1 makes studying SREBP1 isoforms 1a and 1c activation easy. This kit detects SREBP-1a and -1c in nuclear extracts of human, mouse and rat samples.

Contents & Storage

One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated oligonucleotides, positive control cell extract, DTT, Herring Sperm DNA, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions. Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.

BROWSE BY:

TransAM™ SREBP1

Contents & Storage

AVAILABLE PRODUCTS