		Transcriptional Regulation Co-Immunoprecipitation DNA Repair Luciferase Reporter Assay Nuclear Receptor ELISAs Supershift & Gelshift Assays Transcription Factor ELISA Epigenetics & Chromatin Chromatin Assembly Chromatin IP DNA Damage – Phospho-H2AX DNA Methylation Histone Acetyltransferase Activity (HAT) Histone Deacetylase Activity (HDAC) Histone Modification ELISAs Histone Purification Methylated Histones Protein Modification GTPase Activation Assay Kinase Assay Substrate Mitosis Assays Nitric Oxide Quantitation Phospho-Protein Detection Sandwich ELISAs SUMOylation Antibodies, Extracts & Proteins Antibodies Cell Extracts Recombinant Proteins Fluorescence & Labeling Albumin Quantification Cell Viability Assay Fluorescent Dyes & Labeling Immunofluorescence Live Cell Membrane Staining Mitosis Determination Protein and Extract Quantification Protein Labeling & Translocation Sample Preparation Fluorescent Dye Labeling Mitochondrial Separation mRNA Isolation Normalization Nuclear & Cytoplasmic Extraction Protein & Extract Quantification Transfection & Expression Competent E. coli Gene Silencing Protein Transfection Staining Reagents Bioinformatics & Text Mining TimeLogic® Biocomputing Solutions AKS 2

ChIP-IT™ Express HT

maximze your efforts with real high-throughput ChIP

ChIP-IT™ Express HT allows you to perform chromatin IP in a fast, reproducible high-throughput format. It combines the efficiency of the groundbreaking magnetic bead-based ChIP-IT™ Express Kit with a 96-well plate format, enabling the rapid and efficient processing of a large number of ChIP reactions.

Faster plate-based protocol
Process up to 96 ChIP reactions simultaneously
Works with chromatin preparation kits for sonication or enzymatic shearing
Compatible with ChIP-chip or ChIP-seq methodologies

For a limited time, you will receive a free MAG-96 magnetic stand when you purchase ChIP-IT Express HT (while quantities last).

The newest member of the ChIP-IT Express family – ChIP-IT Express HT

ChIP-IT Express HT is based on the improvements made during the development of ChIP-IT Express, the first magnetic bead-based ChIP kit. In ChIP-IT Express and ChIP-IT Express HT, cells are treated with formaldehyde to fix protein/DNA interactions and then the fixed chromatin is sheared by either sonication or enzymatic digestion. The sheared chromatin is incubated with an antibody directed against a protein of interest, and antibody-bound protein/DNA complexes are precipitated through use of magnetic Protein G-coated beads. The captured chromatin is then eluted and cross-links are reversed so that the released DNA is ready for PCR analysis to localize the protein to a specific DNA region or regions (Figure 1).

Figure 1: Schematic of chromatin immunoprecipitation using ChIP-IT Express HT.

Why use ChIP-IT Express HT?

Complete solution – all critical reagents needed are supplied
Compatible with genome-wide profiling or selective PCR-based approaches
No need to optimize reagents and protocol
No phenol/chloroform extractions – DNA purification step eliminated in ChIP-IT Express and ChIP-IT Express HT

Figure 2: Chromatin immunoprecipitation using ChIP-IT Express HT.

PCR analysis of immunoprecipitated DNA is shown. HeLa cells were fixed for 10 minutes with 1% formaldehyde and then chromatin was prepared by sonication shearing (5 pulses). ChIP was performed in duplicate on chromatin using a Negative Control IgG and an RNA pol II antibody. The DNA isolated from these ChIP reactions was then analyzed by 36 cycles of PCR using GAPDH positive control primers. These antibodies and primers are available as the ChIP-IT Control Kit – Human. Kits for mouse and rat are also available (see Available Products, above right). Ten µl of each PCR was separated on a 1% agarose gel and visualized by UV-illumination following ethidium bromide staining. PCR using the GAPDH primers on DNA isolated with the RNA pol II antibody (Lanes 1-4) reproducibly generated more product than similar reactions performed on DNA isolated using the Negative Control IgG (Lanes 5-8). These results demonstrate that ChIP performed with RNA pol II antibody (compared to negative IgG) greatly enriched for GAPDH promoter DNA. Lane 9, No DNA control. Lane 10, Input DNA control.

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