YOUR CART
Active Motif's Histone Purification Mini Kit enables you to purify core histones (H2A, H2B, H3 and H4) as one total population while preserving their post-translational modifications such as acetylation, methylation, and phosphorylation states. This purification method is an improvement over acid precipitation methods and utilizes a convenient spin column and proprietary buffer system to purify the core histones from cells and tissue samples. Histones isolated by this method are suitable substrates for downstream assays and in vitro chromatin assembly.
Advantages
- Convenient spin column format
- Preserves modifications on histones
- Purify histones from as few as 8x105 cells
- Useful for purifying histones from budding yeast
Contents & Storage
5X Neutralization Buffer, Extraction Buffer, Equilibration Buffer, Histone Wash Buffer, Histone Elution Buffer, 20 purification columns and 40 collection tubes. Reagents are to be stored at 4°C. The columns can be stored at room temperature. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.
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Figure 1:Histone Purification Mini Kit spin column in a collection tube. |
