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Epigenetics & Chromatin

Re-ChIP-IT™

localize two different proteins to the same genomic locus

 

When performing chromatin immunoprecipitation (ChIP) experiments, it is often useful to prove that two different proteins or histone modifications are present at the same site in the genome. Or, you may want to determine if a protein coincides with a specific histone modification at the same regulatory element. Re-ChIP (aka Sequential ChIP, Chromatin Re-IP and ChIP Re-ChIP) is a relatively new technique that enables sequential chromatin immunoprecipitations to be performed using two different antibodies so that you can assay for the simultaneous presence of two proteins or distinct histone modifications at the same genomic region of interest.

A complete solution for rapid and efficient Re-ChIP

  • Convenient magnetic bead-based protocol
  • Fewer cells required than other Re-ChIP methods
  • Compatible with multi-channel pipetting
 
Name Format Cat. No. Price  
Re-ChIP-IT™ 25 rxns 53016 $ 595 Buy Now
ChIP-IT™ Shearing Kit for sonication (included in 53001, 53004 & 53008) 10 rxns 53002 $ 165 Buy Now
Enzymatic Shearing Kit (included in 53006, 53007 & 53009) 10 rxns 53005 $ 190 Buy Now
ChIP-IT™ Control Kit – Human 5 rxns 53010 $ 85 Buy Now
ChIP-IT™ Control Kit – Mouse 5 rxns 53011 $ 85 Buy Now
ChIP-IT™ Control Kit – Rat 5 rxns 53012 $ 85 Buy Now
Ready-to-ChIP HeLa Chromatin 10 rxns 53015 $ 315 Buy Now
Ready-to-ChIP Hep G2 Chromatin 10 rxns 53019 $ 315 Buy Now
Ready-to-ChIP K-562 Chromatin 10 rxns 53020 $ 315 Buy Now
Ready-to-ChIP NIH/3T3 Chromatin 10 rxns 53021 $ 315 Buy Now