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When performing chromatin immunoprecipitation (ChIP) experiments, it is often useful to prove that two different proteins or histone modifications are present at the same site in the genome. Or, you may want to determine if a protein coincides with a specific histone modification at the same regulatory element. Re-ChIP (aka Sequential ChIP, Chromatin Re-IP and ChIP Re-ChIP) is a relatively new technique that enables sequential chromatin immunoprecipitations to be performed using two different antibodies so that you can assay for the simultaneous presence of two proteins or distinct histone modifications at the same genomic region of interest.
A complete solution for rapid and efficient Re-ChIP
- Convenient magnetic bead-based protocol
- Fewer cells required than other Re-ChIP methods
- Compatible with multi-channel pipetting
Flexible ordering options
The Re-ChIP-IT Kit provides sufficient components to perform 25 Re-ChIP reactions. The Re-ChIP-IT kit does not include the shearing components or the positive controls found in the ChIP-IT Express Kits. For this reason, the ChIP-IT Sonication & Enzymatic Shearing Kits and Protein G Magnetic Beads are also sold separately. We recommend that you also purchase whichever ChIP-IT Control Kit is appropriate for your sample type, as using proven controls helps in troubleshooting, interpreting your results and validating antibodies for use in ChIP.
Contents & Storage
Protein G Magnetic Beads, LSV Protein G Magnetic Beads, Re-ChIP Purification Columns, ChIP Buffer 1, ChIP Buffer 2, Re-ChIP-IT Elution Buffer, Elution Buffer AM2, Reverse Cross-linking Buffer, Buffered NaCl, 5 M NaCl, Proteinase K, Proteinase K Stop Solution, Protease Inhibitor Cocktail, 10X PCR Buffer, 10X PCR Loading Dye, Bar Magnet, 15 ml Conical Tube, Mini Glue Dots, DEPC-treated Water, Siliconized 1.7 ml microfuge tubes, 0.2 ml PCR stripwell tubes
