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Protein Modification

FACE™ In-cell Phospho ELISAs

simple, efficient analysis of protein phosphorylation

 

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). If you need a FACE Kit for a target that is not currently offered, you can use our FACE Maker Kits  to create your own FACE assays. And, the Suspension Cell FACE module adapts any FACE Kit for improved results when working with suspension cells.

Take 15% Off FACE™ Kits

Through the end of July 2009, you can get 15% off your purchase of any FACE™ Kit in the 1 x 96, 2 x 96 or 3 x 96 rxns sizes. Simply cite the code FACE09 when you place your order. This offer is valid only in the United States and Canada, and only one kit per order qualifies for the discount.

Why use FACE™ Kits?

  • Simple, non-radioactive method that is easily quantified
  • Fixing preserves activation-specific protein modifications
  • Cell-based assay eliminates the need to make cell extracts
  • Minimal hands-on time - less than 3 hours
  • Two primary antibodies to compare phosphorylated and native protein
  • Increased linear analysis range
  • Compatible with high-throughput automation
 
Flow chart of the FACE process of in-cell Westerns for phospho-detection.
 
Figure 1: Flow chart of the FACE process.