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Fluorescence

Cell and Organelle Stains

fluorescent dyes for live or fixed cell imaging

Active Motif offers several products to study cellular structure using fluorescence microscopy or high-content applications. Our Cell and Organelle Stains are a collection of innovative fluorescent dyes that provide specific and efficient staining of subcellular structures in live and in fixed cells. A key feature is that the dyes become fluorescent only after specific binding to target structures; this eliminates background fluorescence caused by any unbound dye. The Cell and Organelle Stains serve as counter-stains in microscopy and can be used as a live cell stain to monitor changes in cell morphology, changes to mitochondrial structure and number, or as a cell-tracer in cell populations. The unique Stem Cell CDy1 Dye accumulates specifically in stem cells, allowing their identification from feeder cells or mixed cultures.

The following table summarizes the fluorescent properties of the organelle-specific dyes, and provides an overview about their performance in different staining experiments.

Cell and Organelle Stain Live cells Fixed cells Long-term staining
Excitation Emission
LavaCell™ Live Cell Membrane Staining ✓* 405, 488 and 532 nm 610 nm
Chromeo™ LT Live Cell Membrane Staining Kit 420-490 nm 510 nm
Chromeo™ Live Cell Mitochondrial Stain 470-550 nm 610 nm
Chromeo™ Red Fluorescent Fixed Cell Staining Kit 520-550 nm 627 nm
Stem Cell CDy1 Dye 480-560 nm 570-660 nm
* While LavaCell is an excellent tool for long-term studies, the dye needs to be kept in the culture medium during the experiment.