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The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. This eliminates the need for optimizing reagents and protocols, and ensures high yields every time. More importantly, standardizing your extraction procedure with our high-quality reagents ensures your reproducible results.
| Name | Format | Cat. No. | Price | |
|---|---|---|---|---|
| Nuclear Extract Kit | 100 rxns | 40010 | $200 |
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| 400 rxns | 40410 | $575 |
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Applications
Extracts prepared using the Nuclear Extract Kit can be used with Active Motif’s TransAM™ Kits, or in other procedures that require cell extracts, like gelshift assays, Western blots, DNA footprinting or as a starting point for transcription factor purification.
Nuclear Extract Kit advantages
- Quality-controlled reagents ensure reproducibility
- No need to optimize your own procedure
- Complete kit contains all required reagents
- Prepare nuclear, cytoplasmic or whole-cell extracts with one kit
Specific extraction
To demonstrate the quality of the materials and protocol of the Nuclear Extract Kit, nuclear and cytoplasmic extracts were prepared from HeLa cell samples that were either unstimulated or stimulated with TNF-α for 30 minutes. Because activated NFκB translocates into the nucleus, only nuclear extract from stimulated cells should contain activated NFκB. As predicted, this is precisely what was seen (Figure 1), demonstrating the kit’s high specificity.
Figure 1: Specific extraction of nuclear and cytoplasmic extracts.
NFκB activation was assayed with the TransAM™ NFκB p50 Kit using increasing amounts of nuclear and cytoplasmic extracts isolated using the Nuclear Extract Kit.
Active Motif also offers a number of ready-to-use nuclear, cytoplasmic and whole-cell extracts. These extracts have been prepared using the same high-quality components found in the Nuclear Extract, and many are supplied as positive controls in TransAM Transcription Factor DNA-binding ELISAs.
Contents & Storage
Lysis Buffer, Dithiothreitol, Protease Inhibitor Cocktail, 10X PBS, Phosphatase Inhibitors, 10X Hypotonic Buffer and Detergent. Reagent storage conditions vary from 4°C to -20°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly
