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Fluorescence

Chromeo™ 488 Tools for STED Microscopy

Chromeo 488 dye and fluorescent secondary antibody conjugates

For STED (STimulated Emission Depletion) microscopy using the Leica TCS STED CW microscope, Active Motif’s fluorescent Chromeo™ 488 dye and conjugated secondary antibodies have been certified by Leica Microsystems. The fluorescent properties of Chromeo 488 dye and secondaries meet the specifications required to perform STED microscopy with  the TCS CW system, which contains a continuous argon gas laser for excitation.

STED microscopy images of cells stained by Nuclear pore protein-1 antibody with Chromeo 488 Goat anti-mouse IgG, and vimentin antibody with Chromeo 488 Goat anti-rabbit IgG
 
Figure 1: Chromeo 488 antibody conjugates in STED microscopy.

Nuclear pore protein-1 (NUP-1) was stained with a primary monoclonal mouse antibody and with Chromeo 488 Goat anti-mouse IgG (Catalog No. 15031) secondary antibody (left). Vimentin was stained with a primary polyclonal rabbit antibody and with Chromeo 488 Goat anti-rabbit IgG (Catalog No. 15041) secondary antibody (right). These STED images are courtesy of Leica Microsystems, Germany.

Chromeo 488 is available as a reactive NHS-Ester or, for convenience in labeling your primary antibodies and proteins, offered in complete, optimized Fluorescent Antibody Labeling Kits. The dye is also available as high-quality anti-mouse and anti-rabbit Fluorescent Secondary Antibody Conjugates that have been validated for use in STED.

To download the STED Microscopy Products Profile, please click here.

Use of Active Motif's primary alpha Tubulin and H3K4me3 antibodies and Chromeo 488 fluorescent secondary antibody in STED microscopy
Figure 2: Active Motif's primary and fluorescent secondary antibodies in STED microscopy.

HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2) (Catalog No. 39527), a biotinylated secondary goat anti-mouse antibody and BD Horizon V500-streptavidin conjugate. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Catalog No. 39159) and Chromeo 488 Goat anti-rabbit IgG (Catalog No. 15041) secondary antibody. The STED image is courtesy of Leica Microsystems, Germany.