Active Motif,
Tools to analyze nuclear function,
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Gene Regulation

TransAM® NFκB

dna-binding ELISAs for activated NFkB transcription factors

TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Active Motif offers multiple TransAM formats in order to meet your research needs when studying NFkB.

Which TransAM® Kit is right for you?

The table below gives a brief overview of the different features of each TransAM Kit format, and provides links to obtain more information.

Kit Format Features Targets
Colorimetric TransAM® NFkB
  • 96-stripwell plate pre-coated with consensus NFkB binding site
  • Works with as little as 2 µg nuclear or 4 µg whole-cell extract
  • Colorimetric change is measured with a microplate spectrophotometer capable of reading at 450 nm

TransAM® NFkB p50

TransAM® NFkB p52

TransAM® NFkB p65

TransAM® NFkB Family
Chemiluminescent TransAM® NFkB Chemi
  • 96-stripwell plate pre-coated with consensus NFkB binding site
  • Works with as little as 0.2 µg nuclear or 0.4 µg whole-cell extract
  • Luminescence is read using a luminometer or CCD camera system

TransAM® NFkB p50 Chemi

TransAM® NFkB p52 Chemi

TransAM® NFkB p65 Chemi
Flexi TransAM® Flexi NFkB
  • Design your own biotinylated oligos to study variant transcription factor-binding sites
  • Bind biotinylated oligos and sample in solution, then transfer to streptavidin coated 96-stripwell plate
  • Colorimetric change is measured with a microplate spectrophotometer capable of reading at 450nm

TransAM® Flexi NFkB p50

TransAM® Flexi NFkB p65

TransAM® Flexi NFkB Family