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Active Motif's Methylated DNA Standard Kit includes three recombinant DNA standards derived from the APC gene promoter: unmethylated DNA, 5-methylcytosine methylated DNA and 5-hydroxymethylcytosine methylated DNA. This kit (which also includes PCR primers specific to the APC promoter) can be used as part of experiments utilizing our antibodies specific to the different types of DNA methylation.
| Name | Format | Cat No. | Price | |
|---|---|---|---|---|
| Methylated DNA Standard Kit | 3 x 2.5 µg | 55008 | $315 | Buy Now |
Figure 1: Agarose gel analysis of Methylated DNA Standard Kit samples.
500 ng of each DNA standard was loaded on a 2.5% agarose gel. Lane 1: unmethylated DNA. Lane 2: 5-methylcytosine containing DNA. Lane 3: 5-hydroxymethylcytosine containing DNA.
Figure 2: Dot blot analysis Methylated DNA Standard Kit samples.
DNA (50 ng) from the three different samples was spotted onto a positively charged nylon membrane. Top panel: Dot blot probed with 5-Methylcytidine antibody (Clone 33D3) (Catalog No. 39649, 1:1,000 dilution). Bottom panel: Dot blot probed with 5-Hydroxymethylcytidine antibody (Catalog No. 39769, 1:5,000 dilution). Lane 1: Single stranded DNA. Lane 2: Double stranded DNA.
Contents & Storage
This kit contains 3 x 2.5 µg each DNA amplified from the APC gene promoter (50 ng/µl) and APC Primer mix (3 x 400 µl each, 2.5 µM concentration).
Store at -20°C. This product is guaranteed stable for 6 months from date of receipt when stored properly.
