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Model Organism Control qPCR Primer Sets

Model Organism Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with samples from rat, zebrafish, Drosophila or budding yeast. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP DNA from multiple samples.

Click the Model Organism qPCR Primer Sets tab below for information on the target of each primer set and what it is an appropriate control for.

 
Name Format Cat No. Price  
Rat Negative Control Primer Set 1 96 rxns 71024 $80 Buy Now
Rat Negative Control Primer Set 2 96 rxns 71025 $80 Buy Now
Rat Positive Control Primer Set Actb 96 rxns 71026 $80 Buy Now
Rat Positive Control Primer Set Gapdh 96 rxns 71027 $80 Buy Now
Drosophila Negative Control Primer Set 1 96 rxns 71028 $80 Buy Now
Drosophila Negative Control Primer Set 2 96 rxns 71029 $80 Buy Now
Drosophila Positive Control Primer Set Act5C 96 rxns 71030 $80 Buy Now
Drosophila Positive Control Primer Set Gapdh1 96 rxns 71031 $80 Buy Now
S. cerevisiae Negative Control Primer Set 1 96 rxns 71032 $80 Buy Now
S. cerevisiae Positive Control Primer Set ACT1 96 rxns 71033 $80 Buy Now
Zebrafish Negative Control Primer Set 1 96 rxns 71034 $80 Buy Now
Zebrafish Negative Control Primer Set 2 96 rxns 71035 $80 Buy Now
Zebrafish Positive Control Primer Set bactin1 96 rxns 71036 $80 Buy Now

Model Organism Chromatin IP Control qPCR Primer Sets

Cat. No.   Name Gene Control for...
71024 Rat Negative Control Primer Set 1 Gene desert on rat chromosome 17 Any transcription factor, RNA pol II and most histone modifications
71025 Rat Negative Control Primer Set 2 Gene desert on rat chromosome 5 Any transcription factor, RNA pol II and most histone modifications
71026 Rat Positive Control Primer Set Actb Exon 6 of rat Actb gene RNA pol II phospho Ser2
71027 Rat Positive Control Primer Set Gapdh Intron 1 of rat Gapdh gene RNA pol II phospho Ser2
71028 Drosophila Negative Control Primer Set 1 Gene desert on Drosophila chromosome 3L Any transcription factor, RNA pol II and most histone modifications
71029 Drosophila Negative Control Primer Set 2 Gene desert on Drosophila chromosome 2R Any transcription factor, RNA pol II and most histone modifications
71030 Drosophila Positive Control Primer Set Act5C Intron 1 of Act5C gene RNA pol II phospho Ser2
71031 Drosophila Positive Control Primer Set Gapdh1 Gapdh promoter H3K4me2, H3K4me3
71032 S. cerevisiae Negative Control Primer Set 1 Intergenic region on chromosome 5 Any transcription factor, RNA pol II and most histone modifications
71033 S. cerevisiae Positive Control Primer Set ACT1 Coding region of ACT1 gene RNA pol II phospho Ser2
71034 Zebrafish Negative Control Primer Set 1 Gene desert on chromosome 3 Any transcription factor, RNA pol II and most histone modifications
71035 Zebrafish Negative Control Primer Set 2 Gene desert on chromosome 7 Any transcription factor, RNA pol II and most histone modifications
71036 Zebrafish Positive Control Primer Set bactin Intron 1 of bactin1 gene

H3K4me3

Contents & Storage

Each control primer set contains both forward and reverse primers in 400 µl of nuclease-free distilled water. The final concentration for each primer is 2.5 µM.

The primer sets are shipped at room temperature and should be stored at -20°C upon receipt. The control primer sets are guaranteed stable for 6 months from date of receipt when stored properly.