EpiShear™ Cooled Sonication Platform
more reproducible results when using any probe sonicator
The EpiShear™ Cooled Sonication Platform greatly increases sample-to-sample reproducibility when shearing chromatin or DNA by enabling you to precisely position the sonicator probe at the exact same depth in each sample every time. The platform, affectionately known as “Big Jack”, can be used on the supplied lab stand base or mounted inside a sound enclosure. It comes with your choice of a tube cooler that fits 1.5-2.0 ml microfuge, 15 ml or 50 ml tubes; the tube coolers keep your sample cold during sonication, so you don’t have to shuttle it to and from an ice bucket to prevent it from overheating.
Figure 1: The EpiShear Cooled Sonication Platform.
|EpiShear™ Cooled Sonication Platform, 1.5 ml||1 platform||53073||$1,695||Buy Now|
|EpiShear™ Cooled Sonication Platform, 15 ml||1 platform||53074||$1,695||Buy Now|
|EpiShear™ Cooled Sonication Platform, 50 ml||1 platform||53075||$1,695||Buy Now|
|Benchtop 1.5 ml Tube Cooler||1 cooler||53076||$150||Buy Now|
|Benchtop 15 ml Tube Cooler||1 cooler||53077||$150||Buy Now|
|Benchtop 50 ml Tube Cooler||1 cooler||53078||$150||Buy Now|
|Sound Enclosure, Large||1 enclosure||53060||$750||Buy Now|
The EpiShear Cooled Sonication Platform was designed to improve the sample-to-sample reproducibility obtained when using probe sonicators by enabling the tip of the probe to be positioned at the same depth in the sample each sonication. The platform has been machined out of stainless steel and aluminum, and includes a hand crank, a height counter and a vertical alignment tool, so once you have determined the optimal settings for your probe and tube type, you can recreate them over and over.
The platform is designed to be used with Active Motif's Tube Coolers, which are available in sizes that fit microfuge, 15 ml and 50 ml tubes. The Tube Coolers keep the sample cold during sonication, so you don't need move the sample to and from an ice bath to keep it cold during sonication. With a programmable sonicator like the EpiShear Probe Sonicator, you can simply set the duration of the On and Off pulses, place the sample in the tube cooler, press Start, then walk away.
Figure 1: The EpiShear Cooled Sonication Platform in use with the EpiShear Probe Sonicator.
Figure 2: Close up view of the EpiShear Cooled Sonication Platform holding a probe in a microfuge tube.
Active Motif's Tube Coolers are an integral part of the EpiShear Cooled Sonication Platform as they both hold the tube precisely and keep the sample cold during sonication. This enables you to take full advantage of the programmable features of Active Motif's EpiShear Probe Sonicator. No longer will you have to repeatedly take the probe out of the sample and place the sample in an ice bucket to prevent it from overheating. You can simply set the duration of the On and Off pulses, and the sample can remain in the cooler for up to 2 hours of sonication.
You have your choice of a cooler that will hold a 1.5-2.0 ml microfuge, 15 ml or 50 ml tube when you purchase the platform, and all 3 coolers are also sold separately (Figure 1). This enables you to shear samples of various sizes, or to keep samples cold on your benchtop during other experiments. The Tube Coolers have many more uses than just sonication.
Figure 1: Tube Coolers to hold 1.5-2.0 ml microfuge, 15 ml and 50 ml tubes.
The EpiShear Cooled Sonication Platform is supplied already mounted on a stainless steel rod. Included is a lab support stand base and a rod adapter that make it easy to set up the platform for use. (Figure 1). Hardware is also included that enables you to place the platform inside Active Motif's large sound enclosure (Figure 2), which will reduce the volume of noise produced by your sonicator by approximately 35 db.
Figure 1: Close up view of the EpiShear Cooled Sonication Platform's support stand base and rod adapter.
Figure 2: The EpiShear Cooled Sonication Platform mounted in Active Motif's large sound enclosure.
The EpiShear Cooled Sonication Platform is supplied with the platform assembly as well as a support stand base and rod adapter on which to mount the platform. Also included is an alignment tool for setting the height of the probe in your samples and your choice of one Tube Cooler that will hold either 1.5-2.0 ml microfuge, 15 ml or 50 ml tubes. (Tube coolers are sold separately if you need more than one size.) Adapters and hex wrenches for mounting the platform in a sound enclosure, such as Active Motif's Large Sound Enclosure (Catalog No. 53060), are also included.