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LightSwitch™ Luciferase Assay Kit

substrate and buffer optimized for use with RenSP luciferase

The LightSwitch™ Luciferase Assay Kit was designed to provide superior results and be easy to use. It utilizes our novel, proprietary LightSwitch Assay Substrate, which was formulated specifically for use with our engineered RenSP luciferase gene. In addition, the kit's Assay Buffer makes possible one-step reagent addition, eliminating the need to perform a separate lysis step. The LightSwitch Assay offers a number of advantages:

  • Quantitative – Achieve high sensitivity and a broad dynamic range
    (see the Data tab below)
  • Convenient – Simply mix reagents, add directly to cells, then read on a luminometer
  • Optimized – Get optimal results with LightSwitch assay reagents, developed specifically for use with our LightSwitch Reporter constructs
  • Flexibility – Use for a small number of samples, or easily scale up to perform thousands of assays
Name Format Cat No. Price  
LightSwitch™ Luciferase Assay Kit 100 assays 32031 $150 Buy Now
1000 assays 32032 $745 Buy Now
Graph showing the dynamic range of signal produced by measuring a dilution series prepared using the LightSwitch Luciferase Assay Kit
Figure 1: Broad, linear dynamic range of signal produced by the LightSwitch Luciferase Assay Kit.

To determine the dynamic range of LightSwitch reagents, cell lysate was prepared from HT1080 cells that had been transfected using FuGENE® HD Transfection Reagent with a LightSwitch Promoter Reporter vector containing the ACTB to drive expression of the RenSP luciferase protein (Product ID S717678). A 12-point, 2-fold dilution series of lysate was prepared and total luminescence was measured using the LightSwitch Luciferase Assay Kit. These results show that the kit produces a linear dynamic range across 5 orders of magnitude of signal.

Contents & Storage

The LightSwitch™ Luciferase Assay Kit contains:

  • Lyophilized 100X LightSwitch™ Assay Substrate (-20°C)
  • LightSwitch™ Substrate Solvent (room temperature)
  • LightSwitch™ Assay Buffer (-20°C)
  • Protocol

All reagents are guaranteed stable for 6 months when stored properly.