LightSwitch™ Dual Assay Kit
luciferase reagents for assays with a co-transfection control
The LightSwitch™ Dual Assay Kit was designed to normalize for variation between transfection replicates in cell lines that are particularly difficult to transfect by utilizing a co-transfection control plasmid. In addition to the reagents found in the LightSwitch Luciferase Assay Kit for detection of the RenSP luciferase found on LightSwitch Reporter vectors, the Dual Assay Kit includes reagents from the BioLux® Cypridina Luciferase Assay Kit developed by New England Biolabs for detecting the Cypridina luciferase produced from the Cypridina TK Control Vector (sold separately).
Because LightSwitch Reporter Vectors and the Cypridina TK Control Vector contain different luciferase genes that catalyze different substrates, there is no cross-reaction between the two signals. As an added advantage, the Cypridina reporter protein is secreted into the culture media while the RenSP reporter protein remains within the cell. Normalizing the LightSwitch signal using the Cypridina reporter signal may decrease variation between transfection replicates in difficult cell lines.
See the white paper “Do I need a co-transfection control?” for additional information on the advantages and disadvantages of using a co-transfection control.
|LightSwitch™ Dual Assay Kit||100 assays||32035||$275||Buy Now|
|Cypridina TK Control Vector||5 µg||32036||$195||Buy Now|
|LightSwitch™ Dual Assay Kit Manual|
|LightSwitch™ Cotransfection White Paper|
|LightSwitch™ Luciferase Assay System Profile|
|Gene Regulation Products & Services Brochure|
|LightSwitch™ miRNA Reference Guide|
|Tools for Disease Research|
Contents & Storage
The LightSwitch™ Dual Assay Kit contains:
- Lyophilized 100X LightSwitch™ Assay Substrate (-20°C)
- LightSwitch™ Substrate Solvent (room temperature)
- LightSwitch™ Assay Buffer (-20°C)
- Lyophilized BioLux® Cypridina Luciferase Substrate (-20°C)
- BioLux® Cypridina Luciferase Substrate Solvent (room temperature)
- BioLux® Cypridina Luciferase Assay Buffer (-20°C)
The kit does not include the Cypridina TK Control Vector.
All reagents are guaranteed stable for 6 months when stored properly.