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Sample Preparation

mTRAP™ Maxi & Midi

isolate more mRNA with gripNAs

 

mTRAP™ Maxi & mTRAP™ Midi Kits isolate high-quality mRNA from mammalian cells and tissue samples. The kits utilize Active Motif’s Poly T gripNA™ Probe*, which has a higher affinity and specificity for mRNA than oligo dT. The result is more mRNA per sample, with much less ribosomal RNA and genomic DNA contamination.

* Covered under U.S. Patent No. 6,962,906. Purchase includes the right to use for basic research only. Other-use licenses available, please contact your local Technical Services dept.

 

The mTRAP Maxi Kit provides all reagents and plasticware required for 6 large-scale mRNA isolations. Each reaction can isolate mRNA from 0.5-2.0 x 108 cells or 0.4-1 gram of tissue and will yield 100-200 µg mRNA, depending on cell type.

The mTRAP Midi Kit provides all reagents and plasticware required for 24 medium-sized mRNA isolations. Each reaction can isolate mRNA from 0.5-1.0 x 107 cells or 50-200 mg tissue and will yield 10-20 µg mRNA, depending on cell type.

 

Figure 1: Flowchart of the procedure for the mTRAP Maxi, Midi and Total Kits.