		Transcriptional Regulation Co-Immunoprecipitation DNA Repair Luciferase Reporter Assay Nuclear Receptor ELISAs Supershift & Gelshift Assays Transcription Factor ELISA Epigenetics & Chromatin Chromatin Assembly Chromatin IP DNA Damage – Phospho-H2AX DNA Methylation Histone Acetyltransferase Activity (HAT) Histone Deacetylase Activity (HDAC) Histone Purification Methylated Histones Protein Modification GTPase Activation Assay Kinase Assay Substrate Mitosis Assays Nitric Oxide Quantitation Phospho-Protein Detection Sandwich ELISAs SUMOylation Antibodies, Extracts & Proteins Antibodies Cell Extracts Recombinant Proteins Fluorescence & Labeling Albumin Quantification Cell Viability Assay Fluorescent Dyes & Labeling Immunofluorescence Live Cell Membrane Staining Mitosis Determination Protein and Extract Quantification Protein Labeling & Translocation Sample Preparation Fluorescent Dye Labeling Mitochondrial Separation mRNA Isolation Normalization Nuclear & Cytoplasmic Extraction Protein & Extract Quantification Transfection & Expression Competent E. coli Gene Silencing Protein Transfection Staining Reagents Bioinformatics & Text Mining TimeLogic® Biocomputing Solutions AKS 2

mTRAP™ Total

isolate more mRNA with gripNAs

mTRAP™ Total Kits isolate large yields of high-quality mRNA from total RNA samples, utilizing Active Motif’s Poly T gripNA™ Probe*, which has a higher affinity and specificity for mRNA than oligo dT. The result is more mRNA per sample, with much less ribosomal RNA and genomic DNA contamination.

* Covered under U.S. Patent No. 6,962,906. Purchase includes the right to use for basic research only. Other-use licenses available, please contact your local Technical Services dept.

The mTRAP Total Kit was created for single-pass enrichment of mRNA from total RNA. Reagents and plasticware are included for 12 mRNA isolations. Each reaction can isolate mRNA from up to 500 µg total RNA and will yield 10-15 µg mRNA.

Figure 1: Flowchart of the procedure for the mTRAP Maxi, Midi and Total Kits.

Figure 2: Pure mRNA from total RNA in one passage.

mRNA was isolated from 500 µg of total RNA in duplicate, quantified by spectrophotometry and run on a 0.8% agarose gel.
Lane 1: 1 kb DNA Ladder
Lanes 2-3: 2 µg mRNA isolated in one pass with the mTRAP Total Kit.

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mTRAP™ Total

isolate more mRNA with gripNAs

Figure 1: Flowchart of the procedure for the mTRAP Maxi, Midi and Total Kits.

Figure 2: Pure mRNA from total RNA in one passage.

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