		Transcriptional Regulation Co-Immunoprecipitation DNA Repair Luciferase Reporter Assay Nuclear Receptor ELISAs Supershift & Gelshift Assays Transcription Factor ELISA Epigenetics & Chromatin Chromatin Assembly Chromatin IP DNA Damage – Phospho-H2AX DNA Methylation Histone Acetyltransferase Activity (HAT) Histone Deacetylase Activity (HDAC) Histone Purification Methylated Histones Protein Modification GTPase Activation Assay Kinase Assay Substrate Mitosis Assays Nitric Oxide Quantitation Phospho-Protein Detection Sandwich ELISAs SUMOylation Antibodies, Extracts & Proteins Antibodies Cell Extracts Recombinant Proteins Fluorescence & Labeling Albumin Quantification Cell Viability Assay Fluorescent Dyes & Labeling Immunofluorescence Live Cell Membrane Staining Mitosis Determination Protein and Extract Quantification Protein Labeling & Translocation Sample Preparation Fluorescent Dye Labeling Mitochondrial Separation mRNA Isolation Normalization Nuclear & Cytoplasmic Extraction Protein & Extract Quantification Transfection & Expression Competent E. coli Gene Silencing Protein Transfection Staining Reagents Bioinformatics & Text Mining TimeLogic® Biocomputing Solutions AKS 2

GAPDH Whole-cell Normalization Kit (for mouse)

optimized protein normalization in a convenient format

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. The GAPDH gene is constitutively expressed at high levels in almost all tissues. Because of this, GAPDH is widely used as a loading control for Northern/Western blots and protein normalization.

Contents & Storage

One or five 96-well assay plates coated with Capture Antibody, Detecting Antibody, Lysis and Wash Buffers, BSA, DTT, Protease Inhibitor Cocktail, Recombinant GAPDH and TMB Substrate Stop Solutions. Reagent storage conditions vary from 4°C to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.

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