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Fluorescence

Additional Clickable Fluorophores

Active Motif's set of clickable fluorescent dyes contains six well-known fluorescent dyes offered as click-reactive azides or alkynes. This set of dyes covers the complete spectrum of visible light, enabling fluorescence detection from 460 nm to 670 nm. Our dyes are compatible with most excitation sources including diode lasers, LEDs, tungsten lamps and xenon arc lamps. The broad range of the set increases the potential and flexibility of bio-orthogonal labeling of complex biological samples.

Advantages of Active Motif's Azides and Alkynes

  • Flexibility
  • Ready to use in click-reactions
  • pH insensitive
  • Low background
  • Photostability – enables multiple exposures and increased exposure time

This set of fluorophores and their use in click-chemistry have been described in the following publication:

 
Name Absorption Emission Format Cat No. Price  
330-Dansyl Azide 336 nm 537.5 nm 1 mg 15314 $95 Buy Now
5 x 1 mg 16314 $365 Buy Now
450-Amino Naphthalimide Azide 432 nm 540 nm 1 mg 15315 $100 Buy Now
5 x 1 mg 16315 $385 Buy Now
450-Amino Naphthalimide Alkyne 433 nm 538 nm 1 mg 15316 $100 Buy Now
5 x 1 mg 16316 $385 Buy Now
520-Bodipy Azide 522 nm 534 nm 1 mg 15317 $120 Buy Now
5 x 1 mg 16317 $465 Buy Now
520-Bodipy Alkyne 522 nm 534 nm 1 mg 15318 $120 Buy Now
5 x 1 mg 16318 $465 Buy Now
550-Red Oxazine Azide 557 nm 621 nm 1 mg 15319 $100 Buy Now
5 x 1 mg 16319 $385 Buy Now
550-Red Oxazine Alkyne 558 nm 625 nm 1 mg 15414 $100 Buy Now
5 x 1 mg 16414 $385 Buy Now
625-Nile Blue Azide 629 nm 669 nm 1 mg 15415 $100 Buy Now
5 x 1 mg 16415 $385 Buy Now
625-Nile Blue Alkyne 633 nm 671 nm 1 mg 15416 $100 Buy Now
5 x 1 mg 16416 $385 Buy Now
651-Blue Oxazine Azide 637 nm 666.5 nm 1 mg 15417 $100 Buy Now
5 x 1 mg 16417 $385 Buy Now
651-Blue Oxazine Alkyne 640 nm 666.5 nm 1 mg 15418 $100 Buy Now
5 x 1 mg 16418 $385 Buy Now
 
Dye Chemical Name Structure
330-Dansyl Azide 1-dimethylamine-N(2-azido-ethyl)
naphthalene-5-sulfonamide
330-Dansyl Azide Dye Structure
450-Amino Naphthalimide Azide 6-(6-amino-2-(2-azidopropyl)1,3-dioxo-
1H-benzo(de)-2(3H)isoquinoline
450-Amino Naphthalimide Azide Dye Structure
450-Amino Naphthalimide Alkyne 6-(6-amino-2-(2-propinyl)1,3-dioxo-
1H-benzo(de)-2(3H)isoquinoline
450-Amino Naphthalimide Alkyne Dye Structure
520-Bodipy Azide 8-(4-azidoethyloxyphenyl)-2,6-diethyl-1,3,5,7-tetramethyl-
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
520-Bodipy Azide Dye Structure
520-Bodipy Alkyne 8-(4-propynyloxyphenyl)-2,6-diethyl-1,3,5,7-tetramethyl-
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
520-Bodipy Alkyne Dye Structure
550-Red Oxazine Azide 1-(3-azido-propoxy)-7-methylamino-
phenoxazin-3-one
550-Red Oxazine Azide Dye Structure
550-Red Oxazine Alkyne 1-(2-propynyl)-7-methylamino-
phenoxazin-3-one
550-Red Oxazine Alkyne Dye Structure
625-Nile Blue Azide N-(5-(3-azidopropylamino)-9H-benzo(a)-phenoxazin-9-ylidene)-
N-methyl-methanaminium chloride
625-Nile Blue Azide Dye Structure
625-Nile Blue Alkyne N-(5-(3-propynylamino)-9H-benzo(a)-phenoxazin-9-ylidene)-
N-methyl-methanaminium chloride
625-Nile Blue Alkyne Dye Structure
651-Blue Oxazine Azide (9-(3-azido-propoxy)-7-piperidin-1-yl-phenoxazin-3-ylidene)-
dimethyl-ammonium perchlorate
651-Blue Oxazine Azide Dye Structure
651-Blue Oxazine Alkyne (9-(3-propinyloxy)-7-piperidin-1-yl-phenoxazin-3-ylidene)-
dimethyl-ammonium perchlorate
651-Blue Oxazine Alkyne Dye Structure
Table 1: Structures of Active Motif Chromeon's clickable fluorescent dyes.

To see a larger structure image, click on the chemical structure of your choice.

 
Dye Absorption Emission Spectra ε L/(mol-cm) Stokes shift
330-Dansyl Azide 336 nm 537.5 nm 330-Dansyl Azide Dye Spectra 3,4000 201.5 nm
450-Amino Naphthalimide Azide 432 nm 540 nm 450-Amino Naphthalimide Azide Dye Spectra 6,800 108 nm
450-Amino Naphthalimide Alkyne 433 nm 538 nm 450-Amino Naphthalimide Alkyne Dye Spectra 6,800 105 nm
520-Bodipy Azide 522 nm 534 nm 520-Bodipy Azide Dye Spectra 63,000 12 nm
520-Bodipy Alkyne 522 nm 534 nm 520-Bodipy Alkyne Dye Spectra 63,000 12 nm
550-Red Oxazine Azide 557 nm 621 nm 550-Red Oxazine Azide Dye Spectra 70,000 64 nm
550-Red Oxazine Alkyne 558 nm 625 nm 550-Red Oxazine Alkyne Dye Spectra 70,000 67 nm
625-Nile Blue Azide 629 nm 669 nm 625-Nile Blue Azide Dye Spectra 18,000 44.5 nm
625-Nile Blue Alkyne 633 nm 671 nm 625-Nile Blue Alkyne Dye Spectra 18,000 43.5 nm
651-Blue Oxazine Azide 637 nm 666.5 nm 651-Blue Oxazine Azide Dye Spectra 73,000 29.5 nm
651-Blue Oxazine Alkyne 640 nm 666.5 nm 651-Blue Oxazine Alkyne Dye Spectra 73,000 26.5 nm
Table 2: Properties of Active Motif Chromeon's clickable fluorescent dyes.

To see a larger spectra image, click on the spectrum of your choice.

Contents & Storage

All fluorescent dye azides and alkynes are supplied lyophilized and should be stored at the recommended temperature in the dark.

All products are guaranteed 6 months when stored properly.

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