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Antibodies

Chromeo™ 488 Goat anti-Mouse IgG

Catalog No: 15031 Format: 1 mg $175 Buy Now
Catalog No: 15051 Format: 100 µg $95 Buy Now
Application:Immunofluorescence
Absorption:498 nm
Emission:524 nm
Molar Extinction:73,000 M-1 cm-1
Quantum Yield:~48%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Mouse
Purification:Affinity Purified

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for Chromeo 488 Goat anti-Mouse IgGContents

Contents: 1 mg (Catalog No. 15031) or 0.1 mg (Catalog No. 15051) of Chromeo™ 488 conjugated Goat anti-Mouse IgG (H+L) and 1 ml MAXfluor™ Mounting Medium. The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.

Chromeo 488 is spectrally similar to FITC and Alexa® 488. The conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching when combined with MAXfluor Mounting Medium. Chromeo 488 conjugated antibodies have absorption and emission maxima of approximately 490 and 527 nm, which are compatible with common excitation sources and filter sets.

for Chromeo 488 Goat anti-Mouse IgGApplication Notes

Applications:
Immunofluorescence: 1:1,000 to 1:2,000 dilution
Plate-based assays: 1:800 to 1:1,000 dilution

Chromeo 488 Goat anti-Mouse IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leica's STimulated Emission Depletion (STED) microscopes. It has also been tested and verified by Leica for use in Ground State Depletion with Individual Molecule return (GSDIM) using the SR GSD microscope. STED enables resolution of 50-70 nm, while GSDIM can mathematically re-construct images with resolution as low as 20 nm.

 
Absorption and emission spectra of Chromeo 488 Dye.

Absorption and emission spectra of Chromeo 488 Dye.

HeLa cells stained with alpha Tubulin mAb and Chromeo 488 Goat anti-mouse IgG

HeLa cells stained with alpha Tubulin mAb and Chromeo 488 Goat anti-mouse IgG.
HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2; Cat. No. 39527) and Chromeo 488 Goat anti-mouse IgG. The nuclei have been counterstained with DAPI.

Multi-color staining of Phospho H2AX and tubulin using Chromeo 488 and Chromeo 642 secondary antibodies

Multi-color staining of Phospho H2AX and tubulin using Chromeo 488 and Chromeo 642 secondary antibodies in untreated and etoposide-treated HeLa cells.
HeLa cells were either left untreated or treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117) and Chromeo 642 Goat anti-rabbit IgG (Cat. No. 15044), while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2) and Chromeo 488 Goat anti-mouse IgG. The nuclei were counter-stained with DAPI, then the three separate images were merged.

for Chromeo 488 Goat anti-Mouse IgGStorage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies, and the MAXfluor Mounting Medium, should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Chromeo 488 Goat anti-Mouse IgGGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Chromeo™ 488 Goat anti-Mouse IgG
 

Data Thumbnails

Absorption and emission spectra of Chromeo 488 Dye.

Absorption and emission spectra of Chromeo 488 Dye.
(Click image to enlarge and see details.)

HeLa cells stained with alpha Tubulin mAb and Chromeo 488 Goat anti-mouse IgG

HeLa cells stained with alpha Tubulin mAb and Chromeo 488 Goat anti-mouse IgG.
(Click image to enlarge and see details.)

Multi-color staining of Phospho H2AX and tubulin using Chromeo 488 and Chromeo 642 secondary antibodies

Multi-color staining of Phospho H2AX and tubulin using Chromeo 488 and Chromeo 642 secondary antibodies in untreated and etoposide-treated HeLa cells.
(Click image to enlarge and see details.)