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Antibodies

Chromeo™ 546 Goat anti-Mouse IgG

Catalog No: 15033 Format: 1 mg $175 Buy Now
Catalog No: 15053 Format: 100 µg $95 Buy Now
Application:Immunofluorescence
Absorption:550 nm
Emission:567 nm
Molar Extinction:96,800 M-1 cm-1
Quantum Yield:~11%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Mouse
Purification:Affinity Purified

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for Chromeo 546 Goat anti-Mouse IgGContents

Contents: 1 mg (Catalog No. 15033) or 0.1 mg (Catalog No. 15053) of Chromeo™ 546 conjugated Goat anti-Mouse IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.

Chromeo 546 is spectrally similar to Cy3®. The conjugated antibodies exhibit superior luminescent properties and stability towards photo bleaching. Chromeo 546 conjugated antibodies have absorption and emission maxima of approximately 545 and 568 nm, which are compatible with common excitation sources and filter sets.

for Chromeo 546 Goat anti-Mouse IgGApplication Notes

Applications:
Immunoflouresence: 1:1,000 to 1:2,000 dilution
Plate-based assays: 1:800 to 1:1,000 dilution

Chromeo 546 Goat anti-Mouse IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using the Leica SR GSD microscope for performing Ground State Depletion with Individual Molecule return (GSDIM) microscopy. GSDIM can mathematically re-construct images with resolution as low as 20 nm.

 
Absorption and emission spectra of Chromeo 546 Dye.

Absorption and emission spectra of Chromeo 546 Dye.

Chromeo 546 staining in HeLa cells.

Chromeo 546 staining in HeLa cells.
HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2; Cat. No. 39527) and Chromeo 546 Goat anti-Mouse IgG.

for Chromeo 546 Goat anti-Mouse IgGStorage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Chromeo 546 Goat anti-Mouse IgGGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Chromeo™ 546 Goat anti-Mouse IgG
 

Data Thumbnails

Absorption and emission spectra of Chromeo 546 Dye.

Absorption and emission spectra of Chromeo 546 Dye.
(Click image to enlarge and see details.)

Chromeo 546 staining in HeLa cells.

Chromeo 546 staining in HeLa cells.
(Click image to enlarge and see details.)