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Antibodies

ATTO 655 (STED) Goat anti-Mouse IgG

Catalog No: 15039 Format: 250 µl $295 Buy Now
Catalog No: 15059 Format: 35 µl $120 Buy Now
Application:Immunofluorescence
Absorption:663 nm
Emission:684 nm
Molar Extinction:125,000 M-1 cm-1
Quantum Yield:~30%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Mouse
Purification:Affinity Purified

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for ATTO 655 (STED) Goat anti-Mouse IgGContents

Contents: 250 µl (Catalog No. 15039) or 35 µl (Catalog No. 15059) of affinity purified goat anti-mouse IgG (H+L) conjugated to ATTO 655 (STED) in PBS, pH 7.4, 0.01% sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography and cross-adsorbed. It reacts with whole molecule mouse IgG and shows minimum cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, rabbit, rat and sheep serum proteins. No cross-reactivity was observed with non-immunoglobulin serum proteins.

Characteristic features of the ATTO 655 (STED) are strong absorption, good fluorescence quantum yield, excellent thermal and photo stability, outstanding ozone resistance, very good water solubility, and very little triplet formation.

for ATTO 655 (STED) Goat anti-Mouse IgGApplication Notes

Application:
Immunofluorescence: 1:1,000 to 1:2,000

ATTO 655 (STED) Goat anti-Mouse IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leica's STimulated Emission Depletion (STED) microscopes. STED enables resolution of 50-70 nm.

 
Absorption and emission spectra of ATTO 655 (STED) Dye.

Absorption and emission spectra of ATTO 655 (STED) Dye.

Immunofluorescence staining using the ATTO 655 (STED) Goat anti-Mouse IgG secondary antibody.

Immunofluorescence staining using the ATTO 655 (STED) Goat anti-Mouse IgG secondary antibody.
HeLa cells stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527) at a 1:500 dilution and ATTO 655 (STED) Goat anti-Mouse IgG secondary antibody at 1:1,000.

for ATTO 655 (STED) Goat anti-Mouse IgGStorage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATTO 655 (STED) Goat anti-Mouse IgGGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

ATTO 655 (STED) Goat anti-Mouse IgG
 

Data Thumbnails

Absorption and emission spectra of ATTO 655 (STED) Dye.

Absorption and emission spectra of ATTO 655 (STED) Dye.
(Click image to enlarge and see details.)

Immunofluorescence staining using the ATTO 655 (STED) Goat anti-Mouse IgG secondary antibody.

Immunofluorescence staining using the ATTO 655 (STED) Goat anti-Mouse IgG secondary antibody.
(Click image to enlarge and see details.)