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Antibodies

ATTO 532 (GSD) Goat anti-Mouse IgG

Catalog No: 15070 Format: 250 µl $295 Buy Now
Catalog No: 15071 Format: 35 µl $120 Buy Now
Application:Immunofluorescence
Absorption:534 nm
Emission:560 nm
Molar Extinction:115,000 M-1 cm-1
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Mouse
Purification:Affinity Purified

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for ATTO 532 (GSD) Goat anti-Mouse IgGContents

Contents: 250 µl (Catalog No. 15070) or 35 µl (Catalog No. 15071) of affinity purified goat anti-mouse IgG (H+L) conjugated to ATTO 532 (GSD) in PBS, pH 7.4, 0.01% sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography and cross-adsorbed. It reacts with whole molecule mouse IgG and shows minimum cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, rabbit, rat and sheep serum proteins. No cross-reactivity was observed with non-immunoglobulin serum proteins.

ATTO 532 (GSD) belongs to a class of fluorescent labels for the orange-red spectral region, like Rhodamine 6G. The dye is very photostable, exhibits a strong absorption and an excellent fluorescence quantum yield. ATTO 532 (GSD) is very soluble in water, allowing conjugations with high dye-to-protein ratios.

for ATTO 532 (GSD) Goat anti-Mouse IgGApplication Notes

Application:
Immunofluorescence: 1:1,000 to 1:2,000

ATTO 532 (GSD) Goat anti-Mouse IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using the Leica SR GSD microscope for performing Ground State Depletion with Individual Molecule return (GSDIM) microscopy. GSDIM can mathematically re-construct images with resolution as low as 20 nm.

 
Absorption and emission spectra of ATTO 532 (GSD) Dye.

Absorption and emission spectra of ATTO 532 (GSD) Dye.

ATTO 532 (GSD) Goat anti-Mouse secondary antibody conjugate in widefield and GSDIM microscopy.

ATTO 532 (GSD) Goat anti-Mouse secondary antibody conjugate in widefield and GSDIM microscopy.
Tubulin was stained with a primary monoclonal mouse antibody and with ATTO 532 (GSD) Goat anti-Mouse IgG, then imaged using widefield (left) and GSDIM (right) microscopes. GSDIM provides much sharper resolution. These images are courtesy of Leica Microsystems, Germany.

for ATTO 532 (GSD) Goat anti-Mouse IgGStorage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATTO 532 (GSD) Goat anti-Mouse IgGGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

ATTO 532 (GSD) Goat anti-Mouse IgG
 

Data Thumbnails

Absorption and emission spectra of ATTO 532 (GSD) Dye.

Absorption and emission spectra of ATTO 532 (GSD) Dye.
(Click image to enlarge and see details.)

ATTO 532 (GSD) Goat anti-Mouse secondary antibody conjugate in widefield and GSDIM microscopy.

ATTO 532 (GSD) Goat anti-Mouse secondary antibody conjugate in widefield and GSDIM microscopy.
(Click image to enlarge and see details.)