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Antibodies

Rhodamine 6G (GSD) Goat anti-Rabbit IgG

Catalog No: 15076 Format: 250 µl $295 Buy Now
Catalog No: 15077 Format: 35 µl $120 Buy Now
Application:Immunofluorescence
Absorption:508 nm
Emission:558 nm
Molar Extinction:116,000 M-1 cm-1
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Rabbit
Purification:Affinity Purified

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for Rhodamine 6G (GSD) Goat anti-Rabbit IgGContents

Contents: 250 µl (Catalog No. 15076) or 35 µl (Catalog No. 15077) of affinity purified goat anti-rabbit IgG (H+L) conjugated to Rhodamine 6G (GSD) in PBS, pH 7.4, 0.01% sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography and cross-adsorbed. It reacts with whole molecule rabbit IgG and shows minimum cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, mouse, rat and sheep serum proteins. No cross-reactivity was observed with non-immunoglobulin serum proteins.

Rhodamine 6G (GSD) belongs to a class of fluorescent labels for the orange-red spectral region. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, and high photo stability.

for Rhodamine 6G (GSD) Goat anti-Rabbit IgGApplication Notes

Application:
Immunofluorescence: 1:1,000 to 1:2,000

The Rhodamine 6G (GSD) Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using the Leica SR GSD microscope for performing Ground State Depletion with Individual Molecule return (GSDIM) microscopy. GSDIM can mathematically re-construct images with resolution as low as 20 nm.

 
Absorption and emission spectra of Rhodamine 6G (GSD) Dye.

Absorption and emission spectra of Rhodamine 6G (GSD) Dye.

Rhodamine 6G (GSD) Goat anti-Rabbit secondary antibody conjugate in widefield and GSDIM microscopy.

Rhodamine 6G (GSD) Goat anti-Rabbit secondary antibody conjugate in widefield and GSDIM microscopy.
Tubulin was stained with a primary polyclonal rabbit antibody and with Rhodamine 6G (GSD) Goat anti-Rabbit IgG, then imaged using widefield (left) and GSDIM (right) microscopes. GSDIM provides much sharper resolution. These images are courtesy of Leica Microsystems, Germany.

for Rhodamine 6G (GSD) Goat anti-Rabbit IgGStorage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Rhodamine 6G (GSD) Goat anti-Rabbit IgGGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Rhodamine 6G (GSD) Goat anti-Rabbit IgG
 

Data Thumbnails

Absorption and emission spectra of Rhodamine 6G (GSD) Dye.

Absorption and emission spectra of Rhodamine 6G (GSD) Dye.
(Click image to enlarge and see details.)

Rhodamine 6G (GSD) Goat anti-Rabbit secondary antibody conjugate in widefield and GSDIM microscopy.

Rhodamine 6G (GSD) Goat anti-Rabbit secondary antibody conjugate in widefield and GSDIM microscopy.
(Click image to enlarge and see details.)