Contents:
5 µg of Recombinant p53 protein and 1X Protein Dilution Buffer (20 mM Tris-HCl pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA and 1 mM DTT. Protein is supplied at 0.5 µg/µl in Protein Dilution Buffer.
Protein Details:
Full-length recombinant p53 protein was expressed in a baculovirus system with a polyhistidine tag and purified by an affinity column in combination with FPLC chromatography.
Protein Applications:
Recombinant p53 is suitable for TransAM™, protein-protein and DNA protein assays. 1 ng is sufficient for a gelshift assay. The standard curve for TransAM p53 was generated using the range of 40-0.625 ng of protein.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
The standard curve for TransAM™ was generated using a range of 40 - 0.625 ng of protein and run on the TransAM™ p53 ELISA Kit.
Storage:
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
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