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for Recombinant p53 proteinContents
5 µg of Recombinant p53 protein and 1X Protein Dilution Buffer (20 mM Tris-HCl pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA and 1 mM DTT. Protein is supplied at 0.5 µg/µl in Protein Dilution Buffer.
for Recombinant p53 proteinApplication Notes
Recombinant p53 is suitable for TransAM®, protein-protein and DNA protein assays. 1 ng is sufficient for a gelshift assay. The standard curve for TransAM p53 was generated using the range of 40 – 0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
for Recombinant p53 proteinProtein Details
Full-length recombinant p53 protein was expressed in a baculovirus system with a polyhistidine tag and purified by an affinity column in combination with FPLC chromatography.
TransAM® standard curve generated using Recombinant p53 protein.
The standard curve for TransAM® was generated using a range of 40 – 0.625 ng of protein and run on the TransAM® p53 ELISA Kit.
for Recombinant p53 proteinStorage
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant p53 proteinGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
