Contents:
2 µg of Recombinant BRCA1 protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.4 µg/µl in Dilution Buffer AM1.
Application Notes:
The wild-type BRCA1 protein (1-1863 residues, accession number NM 007296) was expressed in a baculovirus system with an amino terminal polyhistidine tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
Protein Usage:
Recombinant BRCA1 is suitable for
in vitro function studies including transcription and DNA repair, for protein-protein interaction assays and cell growth assays. 1 ng is sufficient for a gelshift assay in a 20 µl reaction volume; 50 ng is sufficient for reconstituted transcription assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~205 kDa.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Storage:
Some proteins are supplied lyophilized and some are supplied in solution. Lyophilized proteins can be stored at 4°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
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