Contents:
5 µg of Recombinant c-Fos protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.5 µg/µl in Dilution Buffer AM1.
Application Notes:
Recombinant c-Fos was expressed from full length (accession number NM 003131) with an amino terminal polyhistidine tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
Protein Usage:
Recombinant c-Fos is suitable for DNA and protein-protein interaction assays. 100 ng is sufficient for DNA-protein and protein-protein interaction studies. The molecular weight of the protein is ~50 kDa. The standard curve for TransAM™ AP-1 c-Fos was generated using the range of 40-2.5 ng of protein.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
The standard curve for TransAM™ was generated using a range of 40-10 ng of protein and run on the TransAM™ AP-1 c-Fos ELISA Kit.
Storage:
Some proteins are supplied lyophilized and some are supplied in solution. Lyophilized proteins can be stored at 4°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
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