Contents:
4 µg of Recombinant ER protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.2 µg/µl in Dilution Buffer AM1.
Application Notes:
Recombinant ER is specific for the ER alpha subunit and was expressed from full length (accession number NM 000125) with an amino terminal polyhistidine tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
Protein Usage:
Recombinant ER is suitable for DNA and protein-protein interaction assays. 20-100 ng are sufficient for
in vitro transcription assays and 100 ng are sufficient for protein-protein interaction studies. The molecular weight of the protein is ~64 kDa. This protein is not recommended for use in TransAM™.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Storage:
Some proteins are supplied lyophilized and some are supplied in solution. Lyophilized proteins can be stored at 4°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
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