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for Recombinant ER proteinContents
4 µg of Recombinant ER protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.2 µg/µl in Dilution Buffer AM1.
for Recombinant ER proteinApplication Notes
Recombinant ER is suitable for DNA and protein-protein interaction assays. 20-100 ng are sufficient for in vitro transcription assays and 100 ng are sufficient for protein-protein interaction studies. The molecular weight of the protein is ~64 kDa. This protein is not recommended for use in TransAM®. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
for Recombinant ER proteinProtein Details
Recombinant ER is specific for the ER alpha subunit and was expressed from full length (accession number NM 000125) with an amino terminal polyhistidine tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
for Recombinant ER proteinGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
