Contents:
10 µg of Recombinant FXR protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.5 µg/µl in Dilution Buffer AM1.
Application Notes:
Recombinant FXR is isolated from an
E. coli strain that carries the coding sequence of the human FXR under the control of a T7 promoter (accession number U68233). The purified recombinant protein has an amino terminal polyhistidine tag and is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
Protein Usage:
Recombinant FXR is suitable for DNA and protein-protein interaction assays. 20 ng is sufficient for gelshift assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~55 kDa.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Storage:
Some proteins are supplied lyophilized and some are supplied in solution. Lyophilized proteins can be stored at 4°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
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