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Recombinant FXR protein

Aliases: BAR, HRR1, HRR-1, RIP14, NR1H4
Catalog No: 31120 Format: 10 µg $390 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant FXR proteinContents

10 µg of Recombinant FXR protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant FXR proteinBackground

The farnesoid X receptor (FXR) or nuclear receptor subfamily 1, group H, member 4 (NR1H4) is a nuclear receptor that, when activated, translocates to the nucleus where it interacts with certain proteins, including RXR and PPARGC1A, forming heterodimers that bind to hormone response elements on DNA and modulate gene expression. One of the primary functions of FXR is to suppress bile acid synthesis when levels are high by inhibiting the transcription of cholesterol 7 alpha-hydroxylase (CYP7A1) gene that expresses CYP7A1, the rate-limiting enzyme in bile acid synthesis from cholesterol.

for Recombinant FXR proteinApplication Notes

Recombinant FXR is suitable for DNA and protein-protein interaction assays. 20 ng is sufficient for gelshift assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~55 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

for Recombinant FXR proteinProtein Details

Recombinant FXR is isolated from an E. coli strain that carries the coding sequence of the human FXR under the control of a T7 promoter (accession number U68233). The purified recombinant protein has an N-terminal His-Tag and is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.

for Recombinant FXR proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant FXR protein