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for Purified Sp1 proteinContents
2 µg of Purified Sp1 protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.08 µg/µl in Dilution Buffer AM1.
for Purified Sp1 proteinApplication Notes
Purified Sp1 is suitable for in vitro transcription, DNase I protection and gelshift assays. 50-100 ng is sufficient for reconstituted transcription assays and 5-25 ng in a 20 µl volume are sufficient for gelshift assays. The molecular weight of the protein is ~105 kDa. This protein is not recommended for TransAM® assays. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
for Purified Sp1 proteinProtein Details
The natural Sp1 fraction (accession number NM 138473) was purified from HeLa cell nuclear extract by using wheat germ agglutinin affinity chromatography. The purified protein is greater than 95% homogeneous and contains no detectable protease, DNase or RNase activity.
for Purified Sp1 proteinGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
