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for Recombinant AKT3 protein, activeContents
20 µg of Recombinant AKT3 protein in 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, and 50% glycerol, pH 8.5. Protein is supplied at 1 µg/µl.
for Recombinant AKT3 protein, activeApplication Notes
Recombinant AKT3 is suitable for kinase assays. The molecular weight of the protein is ~60.508 kDa. The activity of the protein is ~ 467,100 units/mg with 1 unit defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to synthetic peptide substrate RPRAATF per minute at 30°C. Recommended Enzyme Dilution Buffer: 50 mM Tris pH 7.5, 0.1 mM EGTA, 10 mM DTT and 1 mg/ml BSA Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant AKT3 protein, activeProtein Details
Recombinant human AKT3 kinase is highly active and is suitable for labeling AKT3 kinase substrates. This kinase is phosphorylated at Thr305 and Ser472. The accession number is AF135794. This protein has an amino terminal polyhistidine tag and had been purified by Ni-NTA agarose chromatography to > 95% by SDS-PAGE.
for Recombinant AKT3 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
