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for Recombinant CHK2 protein, activeContents
20 µg of Recombinant CHK2 protein in 50 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, 0.1 mM EGTA, 270 mM sucrose and Protease Inhibitor Cocktail, pH 7.5. Protein is supplied at 0.29 µg/µl.
for Recombinant CHK2 protein, activeApplication Notes
Recombinant CHK2 is suitable for kinase assays. The molecular weight of the protein is ~66 kDa. The activity of the protein is ~ 40,430 units/mg with 1 unit defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to synthetic peptide substrate KKKVSRSGLYRSPSMPENLNRPR per minute at 30°C. Recommended kinase reaction buffer: 40 mM MOPS pH 7.5, 2 mM EDTA Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant CHK2 protein, activeProtein Details
Recombinant human CHK2 kinase is highly active and is suitable for labeling CHK2 kinase substrates. The accession number is BC0004207. This protein has a carboxy terminal polyhistidine tag and had been purified by Ni-NTA agarose chromatography to > 95% by SDS-PAGE.
for Recombinant CHK2 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
