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Recombinant Proteins & Enzymes

Recombinant CK2α1 protein

Aliases: CSNK2A1, CKII, CSNK2A3
Catalog No: 31149 Format: 10 µg $390 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant CK2a1 proteinContents

10 µg of Recombinant CK2α1 protein in 25 mM Tris-HCl, 500 mM NaCl, 1 mM DTT, 500 µM PMSF, and 50% glycerol, pH 8.5. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant CK2a1 proteinApplication Notes

Recombinant CK2α1 is suitable for kinase assays. The molecular weight of the protein is ~45 kDa. The activity of the protein is ~ 1,320,000 units/mg with 1 unit defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to synthetic peptide substrate RRRDDDSDDD per minute at 37°C. Recommended Kinase Reaction Buffer: 20 mM Tris-HCL, 25 mM β-glycerophosphate, 5 mM EGTA, and 1 mM DTT, pH 7.5. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant CK2a1 proteinProtein Details

Recombinant human CK2α1 was expressed in E. coli as a non-fusion protein. The accession number is M55265. The alpha subunit is the catalytic subunit of serine/threonine protein kinase CK2 and is constitutively active. This protein suitable for labeling CK2α substrates. This protein has shown to be extremely salt sensitive and highest activity is obtained without salt. Purification by several chromatography steps to > 99% by SDS-PAGE.

for Recombinant CK2a1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant CK2α1 protein