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for Recombinant p38a protein, activeContents
20 µg of Recombinant p38α protein in 25 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 50% glycerol, pH 8.5. Protein is supplied at 0.4 µg/µl.
for Recombinant p38a protein, activeApplication Notes
Recombinant p38α is suitable for kinase assays. The molecular weight of the protein is ~42.69 kDa. The activity of the protein is ~ 110,700 units/mg with 1 unit defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to myelin basic protein per minute at 30°C. Recommended Kinase Reaction Buffer: 20 mM Tris-HCL, 25 mM β-glycerophosphate, 5 mM EGTA, 1 mM sodium orthovanadate and 1 mM DTT, pH 7.5. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant p38a protein, activeProtein Details
Recombinant human p38α protein was expressed in E. coli as an N-His-fusion protein. The accession number is L35253. This protein was activated by phosphorylation of the purified p38 in vitro with MKK6. Purified by Ni-agarose chromatography to > 95% by SDS-PAGE.
for Recombinant p38a protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
