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for Recombinant ABL1 protein, activeContents
10 µg of Recombinant ABL1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.206 µg/µl.
for Recombinant ABL1 protein, activeApplication Notes
Recombinant ABL1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~74.14 kDa. The activity of the protein is ~ 33 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyAEKY, 5 µg/50 µl, Recombinant ABL1: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant ABL1 protein, activeProtein Details
Recombinant human ABL1 protein was produced using baculovirus infected Sf9 cells. The protein was against amino acids P118-S535, accession number M14752 and N-terminally fused to GST-Factor Xa cleavage site. Purified by GST-agarose affinity purification.
Recombinant ABL1 protein tested by SDS-PAGE gel.
0.5 µg of ABL1 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-ABL1 or anti-GST antibody.
for Recombinant ABL1 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
