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Recombinant Proteins & Enzymes

Recombinant B-RAF protein

Aliases: BRAF, NS7, BRAF1, RAFB1, B-RAF1
Catalog No: 31161 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant B-RAF proteinContents

10 µg of Recombinant B-RAF protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant B-RAF proteinApplication Notes

Recombinant B-RAF is suitable for kinase assays and Western blot. The molecular weight of the protein is ~69.127 kDa. The activity of the protein is ~ 78 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: MEK1-DN, 5 µg/50 µl, Recombinant B-RAF: 25 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant B-RAF proteinProtein Details

Recombinant human B-RAF protein was produced using baculovirus infected Sf9 cells. The protein was against amino acids Gln417-His766, accession number NM_004333.2 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant B-RAF protein, active analyzed by SDS-PAGE gel.

Recombinant B-RAF protein tested by SDS-PAGE gel.
0.5 µg of B-RAF protein was analyzed using SDS/PAGE followed by Western blot with an anti-GST antibody.

for Recombinant B-RAF proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant B-RAF protein
 

Data Thumbnails

Recombinant B-RAF protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant B-RAF protein, active.
(Click image to enlarge and see details.)