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Recombinant BRK protein

Aliases: PTK6
Catalog No: 31162 Format: 10 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant BRK proteinContents

10 µg of Recombinant BRK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRK proteinApplication Notes

Recombinant BRK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~81.79 kDa. The activity of the protein is ~ 42 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 1 µg/50 µl, Recombinant BRK: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant BRK proteinProtein Details

Recombinant human BRK protein was produced using baculovirus infected Sf9 cells. The protein was against amino acids M1-T451, accession number NM_005975 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

Recombinant BRK protein, active analyzed by SDS-PAGE gel.

Recombinant BRK protein tested by SDS-PAGE gel.
0.5 µg of BRK protein was analyzed using SDS/PAGE followed by Western blot with either an anti-BRK or anti-GST antibody.

for Recombinant BRK proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.


Technical Data Sheet

Recombinant BRK protein

Data Thumbnails

Recombinant BRK protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant BRK protein, active.
(Click image to enlarge and see details.)