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for Recombinant BRK protein, activeContents
10 µg of Recombinant BRK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.114 µg/µl.
for Recombinant BRK protein, activeApplication Notes
Recombinant BRK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~81.79 kDa. The activity of the protein is ~ 42 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 1 µg/50 µl, Recombinant BRK: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant BRK protein, activeProtein Details
Recombinant human BRK protein was produced using baculovirus infected Sf9 cells. The protein was against amino acids M1-T451, accession number NM_005975 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant BRK protein tested by SDS-PAGE gel.
0.5 µg of BRK protein was analyzed using SDS/PAGE followed by Western blot with either an anti-BRK or anti-GST antibody.
for Recombinant BRK protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
