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for Recombinant CHK1 protein, activeContents
10 µg of Recombinant CHK1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.095 µg/µl.
for Recombinant CHK1 protein, activeApplication Notes
Recombinant CHK1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~83.93 kDa. The activity of the protein is ~ 9 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb CTF 1.5 µg/50 µl, Recombinant CHK1: 100 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant CHK1 protein, activeProtein Details
Recombinant human CHK1 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-T476, accession number NM_001274.2 and is N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
for Recombinant CHK1 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
