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Recombinant Proteins & Enzymes

Recombinant CHK1 protein

Aliases: CHEK1
Catalog No: 31163 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant CHK1 proteinContents

10 µg of Recombinant CHK1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant CHK1 proteinApplication Notes

Recombinant CHK1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~83.93 kDa. The activity of the protein is ~ 9 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb CTF 1.5 µg/50 µl, Recombinant CHK1: 100 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant CHK1 proteinProtein Details

Recombinant human CHK1 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-T476, accession number NM_001274.2 and is N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

for Recombinant CHK1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant CHK1 protein