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for Recombinant EGFR protein, activeContents
10 µg of Recombinant EGFR protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.112 µg/µl.
for Recombinant EGFR protein, activeApplication Notes
Recombinant EGFR is suitable for kinase assays and Western blot. The molecular weight of the protein is ~91.002 kDa. The activity of the protein is ~ 93 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 10 µg/50 µl, Recombinant EGFR: 40 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant EGFR protein, activeProtein Details
Recombinant human EGFR protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids H672-A1210, accession number NM_005228 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant EGFR protein tested by SDS-PAGE gel.
0.5 µg of EGFR protein was analyzed using SDS/PAGE followed by Western blot with either an anti-EGFR or anti-GST antibody.
for Recombinant EGFR protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
